Summary: This protocol describes a method to assess adipocyte numbers within a specific depot based on their inducible static nail coupon genomic label.By extracting DNA from a complete adipose tissue depot stemming from two transgenic mouse lines (Adipoq-CreERT2 x ROSA26-tdRFP and Ucp1-CreERT2 x ROSA26-tdRFP), the number of adipocytes can be determined based on the quantification of the recombined LoxPRed sites.This highly sensitive system allows for the quantification of white, brown, and brite/beige adipocytes in a spatially unbiased and size-independent manner.
For complete details on the use and execution of this protocol, please dunk crimson tint refer to Moser et al.(2021).